Skip to main content
. 2017 Jan 9;7:40237. doi: 10.1038/srep40237

Figure 4. OA attenuated the high glucose-induced impairment of NO production via PPARδ.

Figure 4

(a) BAECs were pretreated with GSK0660 (1 μM) for 1 h, and then stimulated with OA (10 μM) for 12 h by following normal (5 mM) or high (30 mM) glucose medium for 12 h. NO was detected by using DAF-FM probe (40 × objective). (b) HUVECs were treated as in (a). Protein levels of p-eNOS, eNOS, p-Akt and Akt levels were assessed. (c) Quantification of p-eNOS/eNOS and p-Akt/Akt levels. *P < 0.05 vs. vehicle control, #P < 0.05 vs. OA. (d) HUVECs were pretreated with GSK0660 (1 μM) for 1 h, then exposed to OA (10 μM) for 24 h, protein levels of p-eNOS, eNOS, p-Akt and Akt levels were assessed. (e) Quantification of p-eNOS/eNOS and p-Akt/Akt levels. *P < 0.05 vs. vehicle control, #P < 0.05 vs HG and ※P < 0.05 vs. OA.