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. 2017 Jan 9;7:39604. doi: 10.1038/srep39604

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Copyright © 2017, The Author(s)

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(A) Representative and (B) quantification of Picrosirius red-stained interstitial collagen in the LV of CB+Placebo, STZ+Placebo and STZ+RLX treated mice. Arrow indicates an example of interstitial collagen. Gene expression of (C) connective tissue growth factor (Ctgf), (D) transforming growth factor-β (Tgfb), (E) tumour necrosis factor-α (Tnf) and (F) p22^phox NADPH oxidase subunit (Cyba) in CB+Placebo, STZ+Placebo and STZ+RLX treated LV. Gene expression is normalised to the reference gene Rn18S and presented as mean ± SEM 2^−∆Ct values x 10³ (Ctgf, Tgfb), 10⁴ (Tnf) or 10⁵ (Cyba). n = 6–8 per group. Significantly (^#P < 0.01) different to STZ+Placebo.