Fig. 2.

Expression of WT and PHAII-mutant WNK4 decreases TER. (a) TER was measured on indicated monolayers as described in Methods. Mean ± SEM from three different clones for each construct is shown. ^*, P < 0.0001 vs. matched uninduced monolayer; ^**, P < 0.0001 vs. matched uninduced monolayer and vs. uninduced WT-WNK4 monolayer. The mean TER in the uninduced state (+dox) is expressed as 100% and the induced state (-dox) is indicated as a percentage of this value. (b) Effect of PHAII-mutant WNK4-Q562E on flux of 3-kDa dextran. Monolayers were prepared in the presence (red circles) or absence (blue triangles) of doxycycline, fluorescent dextran was applied to the apical surface, and its appearance in the basolateral media was measured over time.