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. 2017 Jan 9;7:40345. doi: 10.1038/srep40345

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Copyright © 2017, The Author(s)

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Post-confluent 3T3-L1 preadipocytes (day 0) were differentiated in MDI medium with or without 100 μM GN. (A) mRNA levels of C/EBPβ were determined at the indicated times by qPCR, and its protein level was determined at 18 h by western blot. (B) Expression of JMJD2B was determined at the indicated times by qPCR. (C) Expression of C/EBPβ-targeted cell cycle genes was determined at the indicated times by qPCR. The qPCR data are presented as the mean ± SEM of three replicate experiments. *P < 0.05 vs. no GN treatment. Con means no GN treatment.