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. 2016 Nov 21;6(1):111–124. doi: 10.1016/j.molmet.2016.11.009

Figure 1.

Figure 1

Inguinal adipose tissue development is impaired in Adiponectin-Cre; Zfp423loxP/loxP mice. (A)Adiponectin-Cre; Zfp423loxP/loxP (Zfp423-AKO) mice are generated by breeding Adiponectin-Cre transgenic mice to animals carrying floxed Zfp423 alleles (Zfp423loxP/loxP). (B) Relative mRNA levels of Zfp423 and indicated adipocyte genes in fractionated inguinal white adipose tissue (iWAT) adipocytes from 8 weeks-old control and Zfp423-AKO mice. * denotes p < 0.05 from student's t-test. n = 5 mice. (C–F) Representative H&E staining (c, d) and perilipin immunofluorescence staining (e, f) of iWAT sections obtained from 8 weeks-old control mice. Scale bar, 200 μm. (G–J) Representative (g, h) H&E staining and (i, j) perilipin immunofluorescence staining of iWAT sections obtained from 8 weeks-old Zfp423-AKO mice. Scale bar, 200 μm. (K) Western blot analysis of phosphorylated-Akt (p-AKt) and total Akt protein levels in tissue extracts of gonadal WAT (gWAT), iWAT, and liver of 8 weeks-old old control and Zfp423-AKO mice administrated with insulin (1 U/kg). (L) β3-adrenergic receptor induced lipolysis (measured as glycerol release) in ex vivo diced iWAT and gWAT isolated from 8 weeks-old control and Zfp423-AKO mice. *p < 0.05 from two-way ANOVA. n = 6 mice.