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. 2016 Nov 21;6(1):111–124. doi: 10.1016/j.molmet.2016.11.009

Figure 4.

Figure 4

High-fat diet feeding leads to a pathological inguinal WAT expansion in Adiponectin-Cre; Zfp423loxP/loxP mice. (A) Weekly body weight measurements of control and Zfp423-AKO mice fed chow or high-fat diet (HFD) for 16 weeks following weaning. n = 6–8 mice. (B) Adipose depot mass (normalized to body weight) of control and Zfp423-AKO mice after 8 weeks of HFD feeding. *p < 0.05 from student's test. n = 6–8 mice. (C, D) Representative immunofluorescence images of Perilipin (green) and F4/80 (red) expression in iWAT paraffin sections obtained from control and Zfp423-AKO mice after 8 weeks of HFD feeding. Scale bar, 200 μm. (E) Distribution of adipocyte size in control and Zfp423-AKO iWAT after 8 weeks of HFD feeding. * denotes p < 0.05 from two-way ANOVA. n = 6–8 mice. (F) Average adipocyte size of control and Zfp423-AKO iWAT after 8 weeks of HFD feeding. * denotes p < 0.05 from student's test. n = 6–8 mice. (G) Number of crown-like structures (F4/80 positive) in control and Zfp423-AKO iWAT after 8 weeks of HFD feeding. * denotes p < 0.05 from student's test. n = 6–8 mice. (H–I) Relative mRNA levels of macrophage markers (H) and adipocyte-selective genes (I) in control and Zfp423-AKO iWAT after 8 weeks of HFD feeding. * denotes p < 0.05 from student's test. n = 6 mice.