Skip to main content
. 2016 Nov 21;6(1):111–124. doi: 10.1016/j.molmet.2016.11.009

Figure 5.

Figure 5

Obese Adiponectin-Cre; Zfp423loxP/loxP mice are insulin resistant and have increased hepatic steatosis. (A, B) Serum triglyceride levels in control and Zfp423-AKO mice after 8 (A) or 16 weeks (B) of chow or HFD feeding. * denotes p < 0.05 from two-way ANOVA. n = 6–8 mice. (C, D) Representative H&E staining of livers from control (C) and Zfp423-AKO (D) mice after 8 weeks of HFD feeding. Scale bar, 200 μm. (E, F) Representative H&E staining of livers from control (E) and Zfp423-AKO (F) mice after 16 weeks of HFD feeding. Scale bar, 200 μm. (G) Western blot analysis of phosphorylated-Akt (p-AKt) and total Akt protein levels in liver extracts from 8 week HFD-fed control and Zfp423-AKO mice administrated with insulin (2 U/kg). (H) Glucose tolerance test of control and Zfp423-AKO mice after 8 weeks HFD feeding. * denotes p < 0.05 from Two-way ANOVA. n = 6–8 mice. (I) Insulin tolerance test of control and Zfp423-AKO mice after 8 weeks HFD feeding. * denotes p < 0.05 from Two-way ANOVA. n = 6–8 mice.