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. 2016 Nov 21;6(1):111–124. doi: 10.1016/j.molmet.2016.11.009

Figure 7.

Figure 7

Pathological expansion of subcutaneous WAT is sufficient to trigger impaired systemic nutrient homeostasis. (A, B) Representative immunofluorescence images of perilipin (green) and F4/80 (red) expression in sections of gWAT from control and Zfp423-fetalAKO mice after 8 weeks of HFD feeding. Scale bar, 200 μm. (C, D) Relative mRNA levels of macrophage markers (C) and adipocyte-selective genes (D) in gWAT of control and Zfp423-fetalAKO mice after 8 weeks of HFD feeding. * denotes p < 0.05 from student's t-test. n = 6 mice. (E) Western blot analysis of phosphorylated-Akt (p-AKt) and total Akt protein levels in tissue extracts of gWAT, iWAT, and liver of HFD-fed control and Zfp423-fetalAKO mice administrated with insulin (2 U/kg). (F, G) Representative H&E staining of livers from control and Zfp423-fetalAKO mice after 8 weeks of HFD feeding. Scale bar, 200 μm. (H) Serum triglyceride levels in control and Zfp423-fetalAKO mice after 8 weeks of HFD feeding. * denotes p < 0.05 from student's t-test. n = 6 mice. (I) Glucose tolerance test of control and Zfp423-fetalAKO mice after 8 weeks HFD feeding. * denotes p < 0.05 from student's t-test. n = 6 mice. (J) Insulin tolerance test of control and Zfp423-fetalAKO mice after 8 weeks HFD feeding. * denotes p < 0.05 from student's t-test. n = 6 mice. (K) Western blot analysis and quantification of serum adiponectin levels in control and Zfp423-fetalAKO mice fed with chow or HFD for 8 weeks starting at 5 weeks of age. For quantification, intensity of adiponectin band is normalized to that of IgG band. * denotes p < 0.05 from two-way ANOVA. n = 4 mice.