Fig. 1.

Targeting strategy for the generation of β-synuclein KO mice. Exons are represented by numbered black boxes, and positions of strategic restriction enzyme sites are indicated as follows: E, EcoRI; B, BamHI; X, XhoI; V, EcoRV; and M, MfeI. The locations of the outside probes used for Southern blotting are marked in the WT gene structure, and locations of PCR primers used for genotyping are marked in the targeting-vector structure. The diphtheria toxin (DT) and neomycin-resistance gene cassettes (NEO) that were introduced into the targeting vector for negative and positive selection, respectively, are shown as light and dark boxes, respectively. LoxP recombination sites are indicated by gray triangles, and frt recombination sites are indicated by open ovals. Dashed lines indicate insertion of DNA into the WT gene, and dotted lines indicate removal of DNA by flp or cre recombinase.