Skip to main content
. 2016 Dec 26;2016:9872138. doi: 10.1155/2016/9872138

Figure 1.

Figure 1

Expression of PRRs recognized viral RNA and DNA. (a) Total RNA was extracted from human adipose-derived mesenchymal stem cells (hAD-MSCs) and THP1 cell lines (THP1). Relative mRNA levels of PRRs recognized viral RNA nucleic acids, including TLR3, TLR7, TLR8, MDA5, and RIG-I, and RPRs recognized viral DNA nucleic acids, including TLR9, IFI16, DAI, cGAS, and polIII, were examined using real-time PCR by normalizing to GAPDH in the upper panel. The ΔCt values of genes expression in hAD-MSCs were shown in the lower panel. (b) The protein level of PRRs recognized viral nucleic acids in hAD-MSCs and THP1 were determined by Western blot using specific antibodies. β-Actin was used as loading control. (c) Distribution of TLR3, RIG-I, and IFI16. Indirect immunofluorescence staining using specific antibody, respectively, against TLR3, RIG-I, and IFI16 was performed in hAD-MSCs (left panels). The cells were staining with DAPI (middle panels). Images of TLR3, RIG-I, and IFI16 and DAPI staining were merged, respectively (right panels). Data are present as the mean ± SEM of three experiments. Images represent at least three experiments. Scale bar = 20 μm.