Figure 5.

Involvement of IFI16 in HSV60-triggered immune responses. (a) Knockdown of IFI16 at mRNA level. hAD-MSCs were transfected with individual siIFI16 and siCtrl. After 24 h, the expression of IFI16 was determined using real-time PCR. (b) The protein level of knockdown of IFI16. hAD-MSCs were treated as (a) and the expression of IFI16 was determined by Western blot. (c) IFN-α and IFN-β secretion. hAD-MSCs were transfected with each siRNA. After 24 h, the cells were stimulated with poly(I:C) or HSV60 and the cytokine levels of IFN-α and IFN-β in media were measured using ELISA. (d) The expression of antiviral proteins. hAD-MSCs were treated as (c). Antiviral protein levels were determined using Western blot. Western blot images represent at least three experiments. Data are the means ± SEM of three experiments. ^∗ P < 0.05.