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. 2016 Dec 26;2016:9872138. doi: 10.1155/2016/9872138

Table 1.

Primers used for real-time PCR.

Targets genes Primer pairs (5′ → 3′)
Forward Reverse
GAPDH GCACCGTCAAGGCTGAGAAC ATGGTGGTGAAGACGCCAGT
TLR3 CTCAGAAGATTACCAGCCGCC CCATTATGAGACAGATCTAATG
TLR7 TTAACCAATTGCTTCCGTGT GGTGCCCACACtCAATCTG
TLR8 TGTGGTTGTTTTCTGGATTCAA GCTCTCATGGCTTACATGA
MDA5 GTTGAAAAGGCTGGCTGAAAAC TCGATAACTCCTGAACCACTG
RIG-I GTGCAAAGCCTTGGCATGT TGGCTTGGGATGTGGTCTACTC
TLR9 TGTGAAGCATCCTTCCCTGT GAGAGACAGCGGGTGCAG
IFI16 ACTGAGTACAACAAAGCCATTTGA TTGTGACATTGTCCTGTCCCCAC
DAI CAACAACGGGAGGAAGACAT TCATCTCATTGCTGTGTCCC
cGAS CCTGCTGTAACACTTCTTAT TTAGTCGTAGTTGCTTCCT
IFN-α CTTGAAGGACAGACATGACTTTGG GGATGGTTTCAGCCTTTTGGA
IFN-β GCCGCATTGACCATCTATGAGA GAGATCTTCAGTTTCGGAGGTAAC
Mx1 CAGCACCTGATGGCCTATCA ACGTCTGGAGCATGAAGAACTG
OAS1 AGAGACTTCCTGAAGCAGCG GAGCTCCAGGGCATACTGAG
ISG15 GAGAGGCAGCGAACTCATCT CTTCAGCTCTGACACCGACA