Table 1.
Targets genes | Primer pairs (5′ → 3′) | |
---|---|---|
Forward | Reverse | |
GAPDH | GCACCGTCAAGGCTGAGAAC | ATGGTGGTGAAGACGCCAGT |
TLR3 | CTCAGAAGATTACCAGCCGCC | CCATTATGAGACAGATCTAATG |
TLR7 | TTAACCAATTGCTTCCGTGT | GGTGCCCACACtCAATCTG |
TLR8 | TGTGGTTGTTTTCTGGATTCAA | GCTCTCATGGCTTACATGA |
MDA5 | GTTGAAAAGGCTGGCTGAAAAC | TCGATAACTCCTGAACCACTG |
RIG-I | GTGCAAAGCCTTGGCATGT | TGGCTTGGGATGTGGTCTACTC |
TLR9 | TGTGAAGCATCCTTCCCTGT | GAGAGACAGCGGGTGCAG |
IFI16 | ACTGAGTACAACAAAGCCATTTGA | TTGTGACATTGTCCTGTCCCCAC |
DAI | CAACAACGGGAGGAAGACAT | TCATCTCATTGCTGTGTCCC |
cGAS | CCTGCTGTAACACTTCTTAT | TTAGTCGTAGTTGCTTCCT |
IFN-α | CTTGAAGGACAGACATGACTTTGG | GGATGGTTTCAGCCTTTTGGA |
IFN-β | GCCGCATTGACCATCTATGAGA | GAGATCTTCAGTTTCGGAGGTAAC |
Mx1 | CAGCACCTGATGGCCTATCA | ACGTCTGGAGCATGAAGAACTG |
OAS1 | AGAGACTTCCTGAAGCAGCG | GAGCTCCAGGGCATACTGAG |
ISG15 | GAGAGGCAGCGAACTCATCT | CTTCAGCTCTGACACCGACA |