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Stability of the RNA–protein complexes formed in three different extracts at 25°C (Left) and 0°C (Right). Commitment complex formation and chase assays were carried out as described in Materials and Methods. Complex stability was measured by liquid scintillation counter after immunoprecipitation with an antibody against Prp40. The value obtained after a 20-min incubation without unlabeled RNA was arbitrarily set to 100. Values were averaged from at least four experiments.
