Gut hormone concentration following fermentable carbohydrate supplementation or incubation with SCFA in WT and Ffar2−/−mice. Portal vein peptide YY (PYY) (A), portal vein Glucagon-like peptide-1 (GLP-1) (B), colonic PYY (C), and colonic GLP-1 (D) concentrations of male Ffar2−/− and WT littermates fed a high-fat diet supplemented with inulin (HFI) or cellulose (HFC) for 14 weeks (A–B, n = 10–12) or 2 weeks (C–D n = 4–6). Percentage PYY (E) of GLP-1 (F) release from mixed colonic cultures taken from Ffar2−/− and WT littermates controls following incubation with 50 mM SCFA (30 mM acetate, 12.5 mM propionate, 7.5 mM butyrate) (E). Data represent mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001. Statistical differences determined by student's t-test or two-way ANOVA with post-hoc Bonferroni comparisons.