Table 1. General features of the genome.

	Chromosome	pNF1	pNF2
Topology	Circular	Circular	Circular
Length, bp	6,021,225	184,027	87,093
G+C content, %	70.8	67.2	68.4
Copy no.*	1	0.7	1.8
Protein-coding gene	5,674	160	90
Average CDS length, bp	960	963	832
Coding density, %	90.4	83.7	86.0
Function assigned	2,962 (52.2%)	53 (33.1%)	23 (25.6%)
Matched to hypothetical protein	1,533 (27.0%)	37 (23.1%)	26 (28.9%)
No database match	1,179 (20.8%)	70 (43.8%)	41 (45.5%)
rrn operon	3	0	0
tRNA gene	53	0	0

CDS, protein-coding sequences.

^*The copy numbers of pNF1 and pNF2 were estimated from the statistical distribution of random reads between the plasmids and the chromosome.