Figure 6.
Nuclear factor-erythroid 2-related factor-2 (Nrf2) siRNA diminishes the protective effects of IPEC-J2 cells. IPEC-J2 cells were treated with Nrf2 siRNA or control siRNA and incubated for 24 h. Cells were incubated with oregano essential oil (OEO, 10 μg/mL for 24 h) and then incubated with H2O2 (0.8 mM) for 24 h. (a) Cell viability was determined by MTT assay. (b) Intracellular reactive oxygen species (ROS) levels were measured by DCF fluorescence microscopy. Values represent means ± SEM, n = 3, significant at ∗ P < 0.05, ∗∗ P < 0.01.