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. 2017 Jan 1;30(1):35–44. doi: 10.1089/vim.2016.0113

FIG. 4.

FIG. 4.

B lymphocyte activation markers and inhibitory regulation molecules determined by semiquantitative RT-PCR. (A, B) mRNA expression of B cell activation markers CD69, CD86, and TSPAN33 in Hmy2.CIR and transfected Hmy2.CIR cells. (A) Without stimulation. This result showed that in the resting state, the expression of two B lymphocyte activation markers CD86 and TSPAN33 was slightly increased in HBe-Hmy2.CIR cells. (B) After stimulation with CD40L (1.5 μg/mL) and IL-4 (3 ng/mL) for 12 h. This result presented a significant increase of CD86 and TSPAN33 expression in HBe-Hmy2.CIR cells after these cells were activated. (C, D) mRNA expression of B cell inhibitory regulation molecules CD32b and Lyn in Hmy2.CIR and transfected Hmy2.CIR cells. (C) Without stimulation, (D) stimulated with IgM μ chain (2 μg/mL) for 8 h. No significant differences of these two inhibition markers of B lymphocytes were detected between HBe-Hmy2.CIR and GV166-Hmy2.CIR cells. All experiments were repeated thrice independently. Differences between groups were analyzed using the independent samples Student's t-test and considered to be significant if p < 0.05. *p < 0.05, **p < 0.01.