Skip to main content
. 2004 Oct 6;101(41):14955–14960. doi: 10.1073/pnas.0406462101

Fig. 3.

Fig. 3.

Phenotypes induced by Ce-imp-2 RNAi in C. elegans and cholesterol regulation-related pathway. Shown are Nomarski optic longitudinal views of C. elegans phenotypes. (A Upper) Ce-imp-2 RNAi and sel-12 reduced activities have no synergetic effects on lin-12,glp-1 signaling. ISC defect induced by Ce-imp-2 RNAi is observed in sel-12 (ar131) larvae, but pharyngeal anterior bulb is developed normally. (A Lower) Muscle detachment occasionally detected in N2 Ce-imp-2 RNAi worms and normal pharyngeal anterior bulb are shown. (B) Similar ISC phenotypes are detected in Ce-imp-2 RNAi N2 worms and in Ce-lrp-1-deficient worms (Ce-lrp-1 RNAi N2 worms or Ce-lrp-1 deletion strain MH210). (C) Thick cuticle and compressed pharynx in Ce-imp-2 RNAi N2 and MH210 worms. (D) ISC phenotype and thick cuticle caused by cholesterol depletion in N2 worms. (E) Filipin complexes are localized in anterior head region in P0 worms, which were fed with Ce-imp-2 or Ce-lrp-1 dsRNA (Upper, regular light, black arrows indicate ISC; Lower, UV-light picture, white arrows show filipin localization).