FIG. 3.

Controlled cortical impact results in decreased mitochondrial DNA (mtDNA)-encoded transcripts in ipsilateral striatum. Rats were subjected to either sham or controlled cortical impact treatment. PGC-1α, TFAM, COXI, ND1, and NDUFS1 messenger RNA expression were determined by reverse-transcriptase polymerase chain reaction (PCR) using tubulin as a control gene. mtDNA copy number was determined by quantitative PCR, using ND1 for the mtDNA gene and actin for the nuclear control gene. These markers were measured in the ipsilateral striatum at post-operative days 3 (A) and 6 (B). Values reported as mean ± SEM. n > 4; *p < 0.05. COXI, cytochrome c oxidase subunit 1; ND1, NADH dehydrogenase subunit 1; NDUFS1, NADH dehydrogenase (ubiquinone) Fe-S protein 1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; POD, post-operative day; SEM, standard error of the mean; TBI, traumatic brain injury; TFAM, mitochondrial transcription factor A.