Table 1.

Primers Used for PCR

RT-qPCR Primers
Gene	Primer sequence
PGC-1α	Sense	5′-AGGAAATCCGAGCGGAGCTGA-3′
	Antisense	5′-GCAAGAAGGCGACACATCGAA-3′
ND1	Sense	5′-TGAATCCGAGCATCCTACC-3′
	Antisense	5′-ATTCCTGCTAGGAAAATTGG-3′
COXI	Sense	5′-CCTGAGCAGGAATAGTAGGG-3′
	Antisense	5′-AGTGGTACAAGTCAGTTCCC-3′
TFAM	Sense	5′-TGAAGTTCTTACACTGATGGC-3′
	Antisense	5′-CCACGTCATCTAGTAAAGCC-3′
NDUFS1	Sense	5′-AGATGATTTGGGAACAACGG-3′
	Antisense	5′-TAAGGCTTAGAGGTTAGGGC-3′
SOD2	Sense	5′-CAAGGGAGATGTTACAACTCAGG-3′
	Antisense	5′-CTTAGGGCTCAGGTTTGTCCA-3′
UCP2	Sense	5′-GAGATACCAGAGCACTGTCG-3′
	Antisense	5′-GCTCAGTACAGTTGACAATGG-3′
Tubulin	Sense	5′-CTCTCTGTCGACTACGGAAAG-3′
	Antisense	5′-TGGTGAGGATGGAATTGTAGG-3′

qPCR Primers
Gene	Primer sequence
ND1	Sense	5′-TGAATCCGAGCATCCTACC-3′
	Antisense	5′-ATTCCTGCTAGGAAAATTGG-3′
β-actin	Sense	5′-TAAGGAACAACCCAGCATCC-3′
	Antisense	5′-CAGTGAGGCCAGGATAGAGC-3′

miRNA primers
miRNA	Primer sequence
miR-21	RT	5′-CTCAACTGGTGTCGTGGAGTCGGCAATT-3′
	Sense	5′-ACACTCCAGCTGGGTAGCTTATGAGACT-3′
miR-155	RT	5′-CTCAACTGGTGTCGTGGAGTCGGCAATT-3′
	Sense	5′-ACACTCCAGCTGGGTTAATGCTAATTGTG-3′
U6	RT/antisense	5′-TTCACGAATTTGCGTGTCAT-3′
	Sense	5′-CGCTTCGGCAGCACATATAC-3′
Universal antisense	Antisense	5′-TGGTGTCGTGGAGTCG-3′

COXI, cytochrome c oxidase subunit 1; miR/miRNA, microRNA; ND1, NADH dehydrogenase subunit 1; NDUFS1, NADH dehydrogenase (ubiquinone) Fe-S protein 1; PCR, polymerase chain reaction; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; POD, post-operative day; qPCR, quantitative PCR; RT-qPCR, reverse-transcriptase quantitative polymerase chain reaction; RT, reverse transcriptase; TBI, traumatic brain injury; TFAM, mitochondrial transcription factor A.