Figure 2.

Effect of o-VA on IgE-mediated passive cutaneous anaphylaxis. (A, B) The ear skin of mice (n=5/group) was sensitized with an intradermal injection of anti-DNP IgE (0.5 mg/site) for 48 h. o-VA was orally administered at doses of 2, 10, and 50 mg/kg body weight 1 h before the intravenous injection of a DNP-HSA and 4% Evans blue (1:1) mixture. Thirty minutes later, the thickness of both ears was measured, and the ears were collected to measure the dye pigmentation. The dye was extracted as described in the Materials and methods section and detected using a spectrophotometer. (C) Ear thickness was measured with a dial thickness gauge. Each data point represents the mean±SEM of three independent experiments. ^*P<0.05. Dexa: dexamethasone.