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. 2016 Nov 28;38(1):90–99. doi: 10.1038/aps.2016.112

Figure 4.

Figure 4

Effects of o-VA on the expression of pro-inflammatory cytokines and the activation of signaling proteins and NF-κB. RBL-2H3 cells were pretreated with or without o-VA. Extraction and analysis of mRNA/protein were performed as described in the Materials and methods section. (A) The gene expression of pro-inflammatory cytokines was determined with quantitative real-time PCR. (B) The secretion of pro-inflammatory cytokines was measured by ELISA. Each data point represents the mean±SEM of three independent experiments. (C) NF-κB translocation, IκBα degradation and the activation of signal molecules were assayed by Western blot (N-: nuclear, p-: phosphorylated). β-Actin and lamin B were used as a loading control. The band intensity was digitized and normalized to the relative ratio. The band is representative of three independent experiments. *P<0.05. Dexa: dexamethasone.