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. 2016 Nov 28;6(1):73–85. doi: 10.1016/j.molmet.2016.11.010

Figure 2.

Figure 2

Global IP6K1 deletion stimulates adipose tissue browning. A. At 23 °C, CD-KO IWAT appears brownish. B. Immunoblot analyses indicate upregulation of UCP1 and PGC1α proteins CD-KO IWAT and RWAT at 23 °C. C. Immunohistochemistry of UCP1 also reveals browning in CD-KO IWAT and RWAT at 23 °C. D and E. Amplification of the browning and mitochondrial machinery in CD-KO RWAT (n = 5–7 mice/group, t-test) and IWAT (n = 4 mice/group, t-test) at 23 °C. F. RWAT explant isolated from CD-mice at 23 °C displays higher basal and FCCP-induced OCR in the knockouts (n = 4 mice/group, t-test). G. Proton leak is higher in IWAT-beige adipocytes of CD-KO mice (n = 6 mice/preparation, 8 replicates, t-test). H. Carbohydrate induced and maximal oxidative capacity are higher in IWAT-beige adipocytes of CD-KO mice (n = 6 mice/preparation, 8 replicates, t-test). In all panels, data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.