TABLE 1.
Range of peak numbers and sizes in the ARISA profiles of six different environmental communities obtained with the three primer setsa
| Environmental sample | Value for primer set
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| ITSF/ITSReub
|
S-D-Bact-1522-b-S-20/L-D-Bact-132-a-A-18
|
1406F/23Sr
|
|||||||
| No. of peaks ± SDb | Range of peak size (bp) | Range of spacer size (bp) | No. of peaks ± SDb | Range of peak size (bp) | Range of spacer size (bp) | No. of peaks ± SDb | Range of peak size (bp) | Range of spacer size (bp) | |
| Natural soil | 115 ± 6 | 231-1,087 | 109-965 | 55 ± 3 | 300-818 | 114-632 | 0 ± 0 | ||
| Polluted soil | 41 ± 16 | 245-698 | 123-576 | 42 ± 21 | 307-737 | 121-551 | 0 ± 0 | ||
| Urania brine | 36 ± 1 | 218-991 | 96-869 | 12 ± 4 | 285-724 | 99-538 | 3 ± 0 | 593-759 | 272-438 |
| Ensiled corn | 33 ± 1 | 294-1,030 | 172-908 | 29 ± 11 | 318-1,056 | 132-870 | 2 ± 1 | 570-1,088 | 249-767 |
| Goat milk | 34 ± 5 | 229-1,463 | 107-1,341 | 5 ± 2 | 293-603 | 107-417 | 4 ± 1 | 444-746 | 123-425 |
| Certosa of Pavia marble | 8 ± 0 | 228-894 | 106-772 | 3 ± 0 | 300-879 | 114-693 | 1 ± 1 | 579-608 | 258-287 |
a
The spacer sizes were calculated by subtracting the number of nucleotides belonging to 16S and 23S rRNA genes from the peak sizes.
b
Data were calculated from three independent ARISA experiments.