TABLE 3.
Results of ARISA of seven reference strains belonging to different bacterial groups performed with the three tested primer sets
Strain | Phylogenetic group | Result with primer seta
|
||
---|---|---|---|---|
ITSF/ITSReub | S-D-Bact-1522-b-S-20/ L-D-Bact-132-a-A-18 | 1406F/23Sr | ||
A. undicula USDA 4903 | α-Proteobacteria | ++ | ++ | + |
R. leguminosarum USDA 2370 | α-Proteobacteria | ++ | + | − |
S. meliloti USDA 1002 | α-Proteobacteria | ++ | + | − |
E. coli DSMZ 50902 | γ-Proteobacteria | ++ | ++ | ++ |
P. stutzeri 1015 | γ-Proteobacteria | ++ | + | + |
B. cereus DSMZ 31T | Gram positive, low G + C | ++ | ++ | ++ |
S. bovis JM 300 | Gram positive, low G + C | ++ | ++ | ++ |
S. coelicolor M145 | Gram positive, high G + C | ++ | ++ | + |
++, peak height between 1,000 and 7,000 fluorescence units; +, peak height lower than 1,000 fluorescence units; −, no peak detected.