TABLE 2.
FISH rRNA probes developed and used for this study
| Probe namea | Probe sequence (5′-3′) | Length (bp) | Tm (°C)b | GC% | Optimal amt (%) of formamide | Target |
|---|---|---|---|---|---|---|
| Doh299 | TCTCTCCGGTATCGTACCC | 19 | 58 | 58 | 10-25 | IM group II |
| Oil845 | TCGTACGGTGCCGATGGA | 18 | 61 | 65 | 20 | IM group II |
| Eur1108 | TTTAAGGGCCGAGGTCTC | 18 | 58 | 56 | 10 | Eurotiomycetes |
a
Probes were named according to their positions in Escherichia coli.
b
By nearest neighbor calculation (http://paris.chem.yale.edu/extinct.html) using 50 mM NaCl and 50 μM oligonucleotide.