Table 2.
Primers used in this study
| Primer name | Sequence (5′–3′) |
|---|---|
| HAA1_del_fw | ACAGCACCAGCACTTGATTG |
| HAA1_del_rv | ATGGTCTTACTCTCTGATACC |
| HAA1_del_control_fw | ACAGAGTCGTGCATTTCCAC |
| HAA1_del_control_rv | CCATGAGTGACGACTGAATC |
| HAA1_BamHI_fw | CAGTGGATCCCTCTATGAGAAGAACCCACG |
| HAA1_BamHI_rv | CAGTGGATCCTACACAACAAACTACGCAAGG |
| HAA1_epPCR_fw | AAAAAGGAAACAAAAGTATAGAAAAAAAAAACCTAAAAAATAATG |
| HAA1_epPCR_rv | AAACTACAGTTACAGAGAAGCAAGAGACGAAAAGCAAATTTATCA |
| HAA1_C404T_GIN11_fw | CTGGCACAGAAAGCCAAAGAAGAAGCAAGAGCTAAAGCCAATCGGAACCCTAAAGGGAGC |
| HAA1_G1447T_GIN11_fw | TTTACAGATTCATCGTCGATTTCAACGCTTTCCCGTGCAAATCGGAACCCTAAAGGGAGC |
| HAA1_GIN11_rv | GGTACCAGGAAATGAAAGCG |
| HAA1_kanMX_fw | GGGCTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGCAGCTGAAGCTTCGTACGC |
| HAA1_C404T_kanMX_rv | TCAGCTTTCGCTGGCAAGCTTACCGAACTATCTTGCCAGTGCATAGGCCACTAGTGGATCTG |
| HAA1_G1447T_kanMX_rv | CAGCTAGGTTTGAAGGGTCCATCATCATATTTGCTATCGAGCATAGGCCACTAGTGGATCTG |
| HAA1_control_fw | ACAGCACCAGCACTTGATTG |
| GIN11_rv | CTGAAACGCAGATGTGCCTC |
| HAA1_C404T_fw | TGTGAGAGGTGCATAAGAGG |
| HAA1_C404T_rv | CCAATCGTAGACCAAAGAGC |
| HAA1_G1447T_fw | GCTCTTTGGTCTACGATTGG |
| HAA1_G1447T_rv | TGGTTCATCTCGTCTGGTAC |
| HAA1_OE_GIN11_fw | AGCTTCTTTCCACGAAAGAAATAGTGTAAGTTAGAGGTACATCGGAACCCTAAAGGGAGC |
| HAA1_OE_kanMX_rv | ATTAAGAAGCAAATACCTTCCTGTTGCTTGATTTGCCCTGGCATAGGCCACTAGTGGATCTG |
| HAA1_OE_fw | ATGTAGTTCAACTTCTATGAATGCTCGGCGATACGATATGCTCTATGAGAAGAACCCACG |
| HAA1_OE_rv | AAGGCTCATTTCCATGATGGGGTCACAATTATTATCGCACTACACAACAAACTACGCAAGG |
| HAA1_OE_control_fw | GCAATCTCGACGATCAACTG |
| HAA1_OE_control_rv | AAGAACCAGAATGGCAGGAC |
| HAA1_GFP-NAT_fw | CGATCAAGGATTTGCGGATTTGGATAATTTCATGTCTTCGTTA CGG ATC CCC GGG TTA ATT AA |
| HAA1_GFP-NAT_rv | CTACAGTTACAGAGAAGCAAGAGACGAAAAGCAAATTTA TCAGAATTCGAGCTCGTTTAAAC |
| HAA1_STOP483_GFP-NAT_fw | CTTTGACACCGAGTTTTATGGATATTCCCGAAAAAGAAAGA CGG ATC CCC GGG TTA ATT AA |
| HAA1_STOP483_GFP-NAT_rv | CTGTCAGTAATGTAATTGGATGATGGCGATCTTTCCGTTTA TCA GAATTCGAGCTCGTTTAAAC |
| ACT1_RT_fw | CTCCACCACTGCTGAAAGAGAA |
| ACT1_RT_rv | CCAAGGCGACGTAACATAGTTTT |
| HAA1_RT_fw | TCGTGTGGGCGAAGTTAGC |
| HAA1_RT_rv | CCAACCCCATCAATGTCAGAA |
| TPO2_RT_fw | TGAGTGATCAAGAATCTGTTG |
| TPO2_RT_rv | CGGTACGGTTCAATTGCTTT |
| TPO3_RT_fw | TTGTGACTGGCGATCCAGAA |
| TPO3_RT_rv | ACTCCAACGGATCCATGCA |
| YGP1_RT_fw | TGTACAATGTTGCCCGTGTTG |
| YGP1_RT_rv | GGCACCGGCGGATGA |
| YRO2_RT_fw | TGGATCCCAGTCAGAGCAAAGT |
| YRO2_RT_rv | ACCTGGGTGCTCCTTTTGG |
| FPS1_RT_fw | ATCTGGGCCCACGTCTTG |
| FPS1_RT_rv | ATGCACCCAAAGCATTTTATGA |