TABLE 2.
PCR-RFLP profiles, distribution, and sequence identification by partial sequence analysis of the gltA genes of ruminant-infecting Bartonella-reference strains obtained from different species of Hippoboscidae
| PCR-RFLP profile type (associated strain[s]) | Fragment size(s) (bp) after digestion witha:
|
Species of Hippoboscidae flies showing the profile type | No. of samples | No. of sequenced fragments | Closest Bartonella species (% DNA identity based on a fragment of the gltA gene) | ||
|---|---|---|---|---|---|---|---|
| TaqI | DdeI | MseI | |||||
| 1 (B. schoenbuchensis R1) | ND | 300, U | 180, U | L. cervi | 26 | 4 | B. schoenbuchensis R1 (100) |
| M. ovinus | No sequence available | ||||||
| Adult | 1 | ||||||
| Pupa | 3 | ||||||
| 2 (B. chomelii, B. schoenbuchensis | ND | ND | 180, U | L. cervi | 4 | 1 | B. schoenbuchensis R3 (100) |
| R3, and A971) | H. equina | 12 | 1 | B. chomelii (100) | |||
| M. ovinus | A971 cervid strain (99) | ||||||
| Adult | 1 | 1 | |||||
| Pupa | 1 | 1 | |||||
| 3 (B. bovis) | 150, 250 | ND | 150, 220 | None | |||
| 4 (B. capreoli) | ND | 100, 250 | 120, 180 | None | |||
| New (no strain associated) | ND | ND | 200, 180 | L. cervi | 6 | 2 | A971 cervid strain (95) |
| Mixed (profiles 1 and 2) | ND | ND, 300, U | 180, U | L. cervi | 7 | 1 | |
| Clone 1 | B. schoenbuchensis R1 (100) | ||||||
| Clone 2 | B. chomelii (100) | ||||||
a
ND, sample was not digested; U, fragment was of undetectable size.