TABLE 2.
Esterases of LABa
| Organism | Enzyme location | Substrates hydrolyzed
|
Substrates hydrolyzed
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Acetate | Propionate | Butyrate | Valerate | Caproate | Caprylate | Caprate | Laurate | Myristate | Palmitate | Others | ||
| Lactobacillus brevis | CFE | α-N- (71, 280), β-N- (71), o-NP- (71), p-NP- (71, 294)b | α-/β-N- (71, 280), p-NP- (71) | α-N- (71), β-N- (70, 103), p-NP- (71, 294), o-NP- (71) | α-/β-N- (71), p-NP- (71) | α-N- (280), β-N- (103, 280), p-NP- (71, 294) | α-N- (280), β-N- (103), p-/o-NP- (71, 73) | β-N- (103, 280) | β-N- (103) | β-N- (103) | Triacetin (206) | |
| CD | β-N- (103) | β-N- (103) | β-N- (103) | |||||||||
| Lactobacillus buchneri | CFE | α-N- (280), p-NP- (294) | α-/β-N- (280) | α-/β-N- (280), p-NP- (71, 294) | α-/β-N- (280), p-NP- (294) | α-N- (280) | β-N- (280) | p-NP- (294) | ||||
| Lactobacillus casei | CFE | α-N- (71, 141), β-N- (71, 141, 223), o-NP- (70), p-NP- (37, 71, 294) | α-N- (71, 141), β-N- (71), p-NP- (37, 71) | α-N- (71, 141), β-N- (33, 71, 103), p-NP- (33, 37, 71, 73, 156, 294), o-NP- (71, 73) | α-/β-N- (71), p-NP- (71) | β-N- (33, 103), p-NP- (33, 71, 73, 156, 294), o-NP- (71, 73) | β-N- (33, 103), p-NP- (37, 71, 73, 156), o-NP- (71, 73) | p-NP- (37), β-N- (103) | β-N- (33, 103) | β-N- (33, 103), p-NP- (294) | p-NP- (294) | Triacetin (206), Tributyrin (33), unspecified C4 and C8 (216) |
| CD | β-N- (223) | β-N- (103) | β-N- (103) | β-N- (103) | ||||||||
| WC | α-N- (194) | α-N- (227) | ||||||||||
| Lactobacillus curvatus | CFE | p-NP- (294) | β-N- (103), p-NP- (294) | β-N- (103), p-NP- (294) | β-N- (103) | β-N- (103) | β-N- (103) | β-N- (103), p-NP- (294) | ||||
| WC | β-N- (213) | Unspecified C4 and C8 (270) | ||||||||||
| Lactobacillus fermentum | CFE | α-/β-N- (71), p-NP- (71, 294), o-NP- (71) | α-/β-N- (71), p-NP- (71) | α-N- (71), β-N- (71, 103), p-NP- (71, 294), o-NP- (71) | α-/β-N- (71), p-NP- (71) | β-N- (103), p-NP- (71, 294), o-NP- (71) | β-N- (103), p-/o- NP- (71) | β-N- (103) | β-N- (103) | β-N- (103), p-NP- (294) | FAX, methyl ferulate, methyl coumarate (57) | |
| CD | β-N- (106) | β-N- (103, 106) | β-N- (103, 106) | β-N- (103, 106) | β-N- (103, 106) | β-N- (103, 106) | ||||||
| WC | α-N- (194) | Ethyl/methyl ferulate (57), unspecified C4 and C8 (204, 270) | ||||||||||
| Lactobacillus hilgardii | CFE | α-N- (280) | α-N- (280) | α-N- (280) | β-N- (280) | |||||||
| Lactobacillus paracasei | CFE | α-/β-N- (22), p-NP- (294) | α-/β-N- (22) | α-/β-N- (22), p-NP- (294) | α-/β-N- (22), p-NP- (294) | α-/β-N- (22) | α-/β-N- (22) | p-NP- (294) | p-NP- (294) | |||
| WC | Unspecified C4 and C8 (270) | |||||||||||
| Lactobacillus plantarum | CFE | α-N- (71, 141), β-N- (71, 104, 141), p-NP- (71, 294, 303), o-NP- (71) | α-/β-N- (71, 141), p-NP- (71) | α-N- (71, 141), β-N- (71, 103, 104, 141), p-NP- (71, 294, 303), o-NP- (71) | α-/β-N- (71), o-NP- (71) | α-N- (141), β-N- (103, 104), p-NP- (294) | β-N- (103, 104), o-/p-NP- (166) | β-N- (103, 104) | β-N- (103, 104) | p-NP- (294), β-N- (103) | β-N- (104), p-NP- (294) | β-N-Stearate, β-N-oleate, milk fat, tributyrin, tricaprylin, trilaurin (104), triacetin (206) |
| CD | β-N- (103) | β-N- (103) | β-N- (103) | |||||||||
| WC | Tributyrin, oleuropein (153), unspecified C4 and C8 (270) | |||||||||||
| Lactobacillus sakei | WC | β-N- (213) | β-N- (213) | |||||||||
| Pediococcus pentosaceus | CFE | α-/β-N- (19) | α-/β-N- (19) | β-N- (19), p-NP- (281) | ||||||||
| Leuconostoc mesenteroides | CFE | α-/β-N- (141, 280, 281) | α-N- (141, 280) | α-N- (280), β-N- (141, 280) | α-N- (280) | α-N- (280) | Triacetin (206) | |||||
| WC | Tributyrin, oleuropein (153) | |||||||||||
a
Activities are listed on the basis of their cellular location, the ester substrate and, where appropriate, the form of the chromogenic linker. Abbreviations: CFE, cell extract of disrupted cells; CD, cell debris; WC, whole cells in liquid or solid culture; α-N-, α-naphthyl; β-N-, β-naphthyl-; o-NP-, o-nitrophenyl; p-NP-, p-nitrophenyl-; FAX, 2-O-[5-O-(trans-feruroyl)-β-l-arabinofuranosyl]-d-xylopyranose. Data are limited to data derived from species which can be found in association with grape juice or wine.
b
The numbers in parentheses are references.