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. 2017 Jan 9;113(1):81–89. doi: 10.1093/cvr/cvw223

Figure 2.

Figure 2

Ang-1 promotes early atherosclerotic plaque formation in ApoE-/- mice fed a normal chow diet. ApoE-/- mice fed a normal chow diet were infected systemically with adenoviruses encoding Ang-1 (AdAng-1), or control virus (AdEV). The mice were euthanized after 4 weeks of adenovirus treatment and effects on early to intermediate atherosclerotic lesions was investigated. (A) Representative picture of Sudan IV en face (top panel) (n = 4 per group). (B) Sudan IV en face staining was quantified from the beginning of aortic arch to the left common carotid artery using image analysis software and expressed as the percentage lesion area in each vessel. **P < 0.001. (C) Oil red O-positive atherosclerotic areas were calculated by using ImageJ image analysis software (n = 12–15 per group). Atherosclerotic plaques were quantified as described in the legend to Figure 1. *P<0.05. (D) Similarly, three sections from each animal were analysed for MOMA-2-positive lesion size (mm2) (n = 12–15 per group). *P<0.05. Original magnification ×2.5 (Sudan IV en face); bars, 1 mm (Sudan IV).