TABLE 1.
Organism and concn (mM) | pH | ζSmolb (103 V) | EMc (108 m2 · [V · s]−1) | ψ0d (103 V) |
---|---|---|---|---|
C. parapsilosis | ||||
20 | 6.81 | −5.1 | −0.39 ± 0.01 | −6.2 |
40 | 6.84 | −4.5 | −0.35 ± 0.18 | −3.1 |
60 | 6.99 | −3.4 | −0.26 ± 0.11 | −2.1 |
80 | 7.01 | −6.3 | −0.49 ± 0.10 | −1.6 |
100 | 7.03 | −4.7 | −0.37 ± 0.10 | −1.3 |
P. aeruginosa | ||||
20 | 6.95 | −8.0 | −0.62 ± 0.14 | −10.3 |
40 | 6.98 | −6.7 | −0.52 ± 0.07 | −5.3 |
60 | 6.99 | −4.4 | −0.34 ± 0.08 | −3.6 |
80 | 7.01 | −5.4 | −0.42 ± 0.28 | −2.7 |
100 | 7.01 | −3.4 | −0.26 ± 0.10 | −2.2 |
All samples were grown at 37°C until the OD600 was approximately 0.5 and suspended in MES buffer with varying ionic strength to a cell concentration of approximately 108 cells/ml.
Zeta potential as calculated from the Smoluchowski equation.
Average electrophoretic mobility of microbial culture (n = 4) with SDs of repeated data sets.
Surface potential as calculated from soft-particle DLVO theory; this is equivalent to the zeta potential of the solution (36).