TABLE 2.

Kinetic parameters for hydrolysis of various substrates^a

Substrate	kcat (s−1)	Km (mM)	kcat/Km (s−1 mM−1)
α-Cyclodextrin	241 ± 6	2.61 ± 0.17	92.3
β-Cyclodextrin	196 ± 5	2.16 ± 0.11	90.7
γ-Cyclodextrin	173 ± 4	5.12 ± 0.42	33.8
Maltotriose	268 ± 8	62.9 ± 3.8	4.3
Acarbose	228 ± 6	11.1 ± 1.1	20.5
Starchb	67 ± 1	0.52 ± 0.02	128.8

^aKinetic parameters were determined in 50 mM sodium acetate buffer (pH 4.5) at 90°C.

^bK_m was calculated from the number-average molecular weight.