TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Sinorhizobium meliloti | ||
| Rm1021 | SU47/Smr Acd− | 35 |
| Rm5356 | Rm1021 ω5033::Tn5-233, Smr Gmr Spr Acd− | 9 |
| Rm1021(pSP329) | Acd− Smr Tcr | This work |
| Rm1021(pWM2) | Acd+ Smr Tcr | This work |
| Rm11466 | Acd+ Smr Nmr | This work |
| Escherichia coli | ||
| DH5α | SupE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 23 |
| S17-1 | Mobilizing strain with chromosomally integrated RP4 derivative | 45 |
| MT616 | MT607(pRK600), mobilizing strain | 12 |
| Plasmids | ||
| pRK2013 | ColE1 replicon containing RK2 transfer region, Kmr | 11 |
| pRK600 | pRK2013 npt::Tn9, Cmr | 12 |
| pSP329 | RP4 replicon, broad-host-range vector, Tcr | 7 |
| pGS220 | Tn5 carried by pBR322 derivative, Nmr Apr | 10 |
| pWM1 | pUC18 containing the acdS and IrpL genes of R. leguminosarum bv. viciae 128C53K on a 4-kb DNA BamHI fragment | 32 |
| pWM2 | pSP329 containing the acdS and lrpL genes of R. leguminosarum bv. viciae 128C53K, Tcr | 32 |
| pWM3 | pGS220 containing the acdS and lrpL genes of R. leguminosarum bv. viciae 128C53K on a 4-kb DNA fragment | This work |
a
Sm, streptomycin; Gm, gentamicin; Sp, spectinomycin; Tc, tetracyeline; Nm, neomycin; Km, kanamycin; Cm, chloramphenicol; Ap, ampicillin.