TABLE 1.
Phylogenetic affiliations and numbers of bacterial 16S rRNA clones retrieved in libraries generated from density-resolved nucleic acidsa
| Phylogenetic group | No. of clones
|
Characteristicb T-RFs (bp) | |
|---|---|---|---|
| X3Ba (“heavy”)c | X9Ba (“light”)c | ||
| δ-Proteobacteria | |||
| Syntrophobacter spp. | 14 | 69, 70, 167 | |
| Smithella spp. | 5 | 510 | |
| Geobacter spp. | 1 | 6 | 131 |
| WCH1B-27 group | 1 | 163 | |
| Firmicutes | |||
| Bacilli | 1 | 10 | 146 |
| Clostridia | 5 | 50 | 196, 207, 217, 273, 298, 513, 519 |
| Pelotomaculum spp. | 10 | 151 | |
| Desulfosporosinus spp. | 1 | 142 | |
| Selenomonas spp. | 1 | 300 | |
| Syntrophomonas spp. | 3 | 302 | |
| Actinobacteria | 1 | 136 | |
| Acidobacteria | 1 | ? | |
| Chlorobi | 1 | 219 | |
| Bacteroidetes | 2 | 140 | |
| Clone T78 group | 1 | 4 | 516 |
| Candidate division BRC-1 | 1 | 494 | |
| Candidate division OP-10 | 1 | 1 | 165, 305 |
| Unidentified | 1 | 136 | |
a
Characteristic T-RFs for different clone groups are given.
b
T-RFs were detected for a major group of clones within a lineage. T-RFs detected for more than one clone within a group are indicated in boldface.
c
13C enrichment of rRNA.