Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of phloretin hydrolase from E. coli DH5α(pPH3)
| Purification step | Total activity (U) | Total protein (mg) | Sp act, (U mg of protein−1) | Purification factor (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Cell extract | 166.0 | 252 | 0.7 | 1 | 100 |
| Dissolved (NH4)2SO4 precipitate (55-60% saturation) | 25.7 | 24 | 1.1 | 1.7 | 15.5 |
| DEAE Sephacel | 3.3 | 1.6 | 2.1 | 3.2 | 2.0 |
| Phenyl Sepharose | 1.5 | 0.17 | 8.7 | 13.2 | 0.9 |
