TABLE 1.
Toxicity of Cry1Aa α4 mutants toward M. sexta larvae
| Toxin | % Mortalitya at:
|
||
|---|---|---|---|
| 50 ng/ml | 2 μg/ml | 50 μg/ml | |
| Cry1Aa | 45 ± 1 | —b | — |
| R127E | 6 ± 1 | 89 ± 2 | — |
| R127N | 22 ± 1 | 97 ± 1 | — |
| E128C | 24 ± 3 | 92 ± 2 | — |
| E129C | — | 7 ± 2 | 14 ± 2 |
| E129K | — | 7 ± 2 | 8 ± 1 |
| R131D | — | 8 ± 1 | 23 ± 2 |
| R131E | 7 ± 2 | 92 ± 1 | — |
| R131H | — | 6 ± 2 | 7 ± 2 |
| R131Q | 7 ± 1 | 33 ± 1 | 95 ± 2 |
| D136C | — | 7 ± 1 | 9 ± 1 |
| D136N | — | 6 ± 2 | 17 ± 1 |
| D136Y | — | 5 ± 2 | 8 ± 2 |
| T142D | — | 5 ± 2 | 23 ± 1 |
| T143D | - | 10 ± 1 | 21 ± 1 |
a
Values are means ± SEM of five independent experiments.
b
—, not tested.