TABLE 2.

Kinetic parameters for penicillin G conversion^a

Strain	Km (mM)	kcat (s−1)	kcat/Km (M−1 s−1)
S. ambofaciens	NDb	ND
S. chartreusis	12.4 ± 1.43	0.0333 ± 0.0018	2.7
S. clavuligerus	2.58 ± 0.13	0.0453 ± 0.0004	18
F42	0.76 ± 0.05	0.026 ± 0.0006	34
F58	0.45 ± 0.02	0.023 ± 0.0002	51
FF1	0.30 ± 0.004	0.0461 ± 0.0004	154
FF8	0.014 ± 0.008	0.0297 ± 0.0008	2,121

^aEach kinetic parameter is expressed as the mean ± standard error for three independent experiments.

^bND, not determined.