TABLE 2.

Electroporation and lightning-induced transformation frequencies of bacterial isolates and controls with the plasmid pBHC^a

Strain	Electrotransformation frequency		Lightning-induced transformation frequencyb
	Without sucrose (22°C)	With 0.5 M sucrose (4°C)	Experiment 1 (20°C)	Experiment 2 (20°C)
Pseudomonas sp. strain Ee2.2	4.9 × 10−4 (1.7 × 10−4)	2.7 × 10−3 (1.5 × 10−3)	1.2 × 10−5	5.3 × 10−7 (2.8 × 10−7)
Pseudomonas sp. strain N3	2.7 × 10−4 (2.2 × 10−4)	1.9 × 10−2 (1.1 × 10−3)	5.3 × 10−4 (2.8 × 10−4)	7.2 × 10−6
E. coli DH10B	3.6 × 10−4 (2.7 × 10−4)	1.4 × 10−6 (8.5 × 10−7)	<10−9	ND
P. fluorescens C7R12	<10−8	10−7c	<10−9	ND

^aStandard deviations are shown in parentheses. ND, not determined.

^bThere were 5 months between experiments 1 and 2.

^cMgCl₂ (10 mM) was required.