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Primers used for PCR analyses in this study
| Primer | Sequence (5′-3′) | Position | Restriction site |
|---|---|---|---|
| Gh9ISEcoR | GAAGAAATGGAACGCTC | Near EcoRI sitea | |
| ISS1F | TGTGATTATTGTCGCTGTTGG | Upstream in ISS1a | |
| sk1orf16F | GCTCTAGAATAGAAAAAGAATCACGACAAb | 13117 in sk1c | XbaI |
| sk1orf20R | AACTGCAGAAAGTATGAGCGACAACTCTCb | 15469 in sk1 | PstI |
In the vector pGh9:ISS1.
The underlined sequences are restriction sites.
The GenBank accession number for sk1 is AF011378.
