TABLE 3.

RT-PCR analysis of the L. lactis IL-1403 region containing ycaG, rgpE, ycbB, and ycbC

Positions of primer pairs in IL-1403a	GenBank accession no.	Genes	PCR productb	Size of PCR product (kbp)c
421 and 4838	AE006257	ybjB and rmlC	−
1343 and 3008	AE006257	rmlA and ybjF	+	1.7
2822 and 4838	AE006257	ybjF and rmlC	+	2.0
3563 and 1260	AE006257/58	rmlB and rgpA	+	2.9
212 and 2657	AE006258	rgpA and rgpC	+	2.5
2314 and 5575	AE006258	rgpC and ycaG	+	3.2
2314 and 5753	AE006258	rgpC and rgpE	+	3.4
6481 and 7855	AE006258	rgpE and rgpF	+	1.3
6637 and 9866d	AE006258	rgpF and ycbA	+	3.2
8476 and 1875	AE006258/59	ycbA and ycbD	+	3.8
883 and 5344	AE006259	ycbC and ycbG	+	4.4
4973 and 9013	AE006259	ycbG and tagD1	+	4.0
7811 and 9318	AE006259	ycbJ and yccB	+	1.5
7811 and 992	AE006259/60	ycbJ and guaB	−

^aThe numbers are the positions of the first nucleotides in the 5′ ends of the primers used with DNA sequences of L. lactis IL-1403. The number system is the system for the previously published sections of the L. lactis IL-1403 genome. The primers were 20 bp long.

^b−, no PCR product; +, PCR product evident.

^cThe results were obtained by performing overlapping RT-PCR with total RNA from the integrants of L. lactis IL-1403.

^dThe combination was tested with wild-type IL-1403 total RNA, and negative results were obtained.