TABLE 1.
Plasmids constructed in this work
| Plasmid | Relevant characteristics (reference) | Source |
|---|---|---|
| pMec1 | 0.3-kb PCR-fragment obtained with CCDb and CCDe primers cloned into pGEM-T Easy; contains part of dccA | Total DNA |
| pMec2 | 1.5-kb SalI-fragment in pUC28 (5), contains part of the chlorocatechol degradation cluster II | Cosmid 9H11 |
| pMec4 | 2.3-kb SalI-fragment in pUC28, contains part of the chlorocatechol degradation cluster I | 11D11 |
| pMec5 | 0.3-kb PCR-fragment obtained with RDPin_f and RDPin_r primers; contains part of rdpA | Total DNA |
| pMec7 | dccAI in pGEM-T Easy | pMec4 |
| pMec9 | dccAI in pRSET6α (NdeI-BamHI cloned) (46) | pMec7 |
| pMec10 | 2.8-kb NcoI-fragment in pUC28; contains rdpA and a transposase | 10A12 |
| pMec12 | 4.5-kb BamHI-fragment in pUC28; contains part of the chlorocatechol degradation cluster II | 9H11 |
| pMec14 | 0.7-kb PCR-fragment in pGEM-T Easy, contains rdpA | pMec10 |
| pMec16 | 5-kb KpnI-fragment in pUC28; contains sdpA | 10A12 |
| pMec18 | 0.7-kb PCR-fragment in pGEM-T Easy, contains sdpA | pMec16 |
| pMec20 | dccAII in pGEM-T Easy | pMec12 |
| pMec21 | dccAII in pET8c (46) | pMec20 |
| pMec23 | 1.1-kb PCR-fragment obtained with primers tfdBup and tfdBlow cloned into pGEM-T Easy, contains part of tfdB | total DNA |
| pMec25 | 5.5-kb SalI-fragment in pUC18Not; contains tfdB | 11D11 |