TABLE 4.
DccAI and DccAII activities with different substrates measured in cell extract of E. coli BL21(DE3)(pLysS) harboring pMec9 or pMec21
| Substrate | % Activity ± standard deviationa
|
|
|---|---|---|
| DccAI | DccAII | |
| Catechol | 23 ± 2 | 19 ± 3 |
| 3-Chlorocatechol | 41 ± 2 | 34 ± 2 |
| 4-Chlorocatechol | 25 ± 1.6 | 22 ± 0.7 |
| 3-Methylcatechol | 62 ± 3 | 47 ± 3 |
| 4-Methylcatechol | 100 (1.48-2.59) | 100 (1.12-2.15) |
| 3,5-Dichlorocatechol | 56 ± 4 | 49 ± 3 |
| 4,5-Dichlorocatechol | 0.7 ± 0.3 | 0.6 ± 0.3 |
| Tetrachlorocatechol | <0.01 | <0.01 |
| p-Nitrocatechol | <0.01 | <0.01 |
| Protocatechuate | <0.01 | <0.01 |
| Hydroquinone | <0.01 | <0.01 |
a
Percent enzyme activities ± standard deviation compared to the value obtained with 4-methylcatechol. The data represent means of three independent cell extracts, of which each was measured three times with all substrates, except for tetrachloro- and p-nitrocatechol, protocatechuate, and hydroquinone, which represent means of two independent cell extracts. The values in parentheses represent the range of specific activity in the cell extracts (in units per milligram of protein).