TABLE 2.
Overview of 16S rRNA gene sequence types from a clone library of North Sea surface water and from libraries of sorted cells after hybridization with probes EUB338, BET42a, and CF319a
| Accession no. | Closest relative in GenBank database (accession no.) | % Similarity | Phylogenetic affiliation | Library(ies)a |
|---|---|---|---|---|
| AJ630664-AJ630667 | Pelagibacter ubique (AF510191) | 98-99 | SAR11 cluster, α-proteobacteria | a,b,c,d |
| AJ630668-AJ630670 | Clone OM188 (U70687) | 98-99 | a,b,c | |
| AJ630671 | Clone OM155 (U70686) | 96 | b | |
| AJ630672, AJ630673 | Clone MB11B07 (AY033299) | 99 | a,b | |
| AJ630674-AJ630676 | Clone MB11F01 (AY033309) | 98-99 | b,c,d | |
| AJ630677 | Clone Arctic 97A-7 (AF353236) | 97 | b | |
| AJ630678 | Clone OM143 (U70685) | 99 | Rhodobacteriales, α-proteobacteria | a |
| AJ630679 | Roseobacter sp. strain SKA44 (AF261065) | 95 | a | |
| AJ630680 | Roseobacter sp. strain NAC1-19 (AF245628) | 99 | a | |
| AJ630681 | Roseobacter sp. strain NAC11-7 (AF245635) | 99 | c | |
| AJ630682, AJ630683 | Maricaulis salignorans (AJ227806) | 88-89 | a,c | |
| AJ630684, AJ630685 | Clone OCS126 (AF001638) | 92-99 | SAR116, α-proteobacteria | a,b |
| AJ704979, AJ704980, AJ630748 | Clone OM43 (U70704) | 99 | Methylophilales, β-proteobacteria | a,b,c |
| AJ704981, AJ704982 | Burkholderia kurkuriensis (AB024310) | 92 | Burkholderiales, β-proteobacteria | a,b |
| AJ704983 | Variovorax sp. strain WDL1 (AF538929) | 97 | c | |
| AJ704984, AJ630749, AJ704985 | Nitrosospira sp. strain Nsp57 (AY123791) | 93-97 | Nitrosomonadales, β-proteobacteria | b,c,d |
| AJ630686 | Pseudoalteromonas sp. strain ANT6 (AF025576) | 99 | Alteromonadales, γ-proteobacteria | a |
| AJ630687-AJ630689 | Pseudoalteromonas sp. strain SUR560 (AB038036) | 98-99 | a,c,d | |
| AJ630690, AJ630691 | Pseudoalteromonas sp. strain KMM3562 (AY387858) | 97-99 | a,d | |
| AJ630692, AJ630693 | Marinobacter sp. strain 2sq31 (AJ429499) | 92-98 | a,b | |
| AJ630694 | Marinobacter sp. (AJ567576) | 90 | b | |
| AJ630695 | Colwellia sp. (AF424148) | 98 | c | |
| AJ630696 | Shewanella algae (X81622) | 91 | c | |
| AJ630697 | Moraxella osloensis (AF005190) | 99 | Pseudomonadales, γ-proteobacteria | c |
| AJ630698 | Oleispira antarctica (AJ426420) | 96 | Oceanospirillales, γ-proteobacteria | a |
| AJ630699 | Marinomonas protea (AJ238597) | 98 | a | |
| AJ630700 | Oleiphilus messinensis (AJ295154) | 96 | a | |
| AJ630701 | Halomonas sp. strain mp1 (HSP551115) | 89 | b | |
| AJ630702, AJ630703 | Clone OCS5 (AF173975) | 97-99 | SAR86, γ-proteobacteria | a,b |
| AJ630704, AJ630705 | Clone OCS44 (AF001650) | 98-99 | a,b | |
| AJ630706, AJ630707 | Clone MB12G02 (AY033328) | 98-99 | b,d | |
| AJ630708-AJ630710 | Clone HTCC2188 (AY386344) | 93-98 | OM182 clade, γ-proteobacteria | a,b,c |
| AJ630711 | Clone HTCC2121 (AY386341) | 99 | SAR92 clade, γ-proteobacteria | c |
| AJ630712, AJ630713 | Cycloclasticus spirillensus (AY026915) | 93-97 | Thiotrichales, γ-proteobacteria | a,c |
| AJ630714 | Dechloromarinus chlorophilus (AF170359) | 90 | γ-Proteobacteria | a |
| AJ630715 | Clone PLY-P3_21 (AY354852) | 97 | γ-Proteobacteria | c |
| AJ630716, AJ630717 | Clone Arctic97A-13 (AF354618) | 98 | Sphingobacteriales, CFB group | c,d |
| AJ630718, AJ630719 | Clone MB11E04 (AY033305) | 98-99 | c,d | |
| AJ630720 | Clone OM273 (U70709) | 89 | d | |
| AJ630721 | Uncultured Polaribacter sp. (AF354621) | 96 | Flavobacteriales, CFB group | d |
| AJ630722, AJ630723 | Gelidibacter sp. (AF513398) | 89-97 | a,d | |
| AJ630724 | Flavobacterium sp. (U85888) | 97 | d | |
| AJ630725 | Flavobacterium frigridarium (AF162266) | 99 | d | |
| AJ630726, AJ630727 | Crocinitomix catalasitica (AB078042) | 91-95 | b,d | |
| AJ630728 | Clone BY71 (AJ298380) | 95 | Bacteroidetes, CFB group | c |
| AJ630729-AJ630731 | Clone PLY-P2-42 (AY354738) | 98-99 | a,c,d | |
| AJ630732, AJ630733 | Clone PLY-P1-77 (AY354779) | 99 | c,d | |
| AJ630734 | Clone EBAC40 (AF268229) | 97 | b | |
| AJ630735 | Clone 1A5 (AY274868) | 99 | b | |
| AJ630736 | Clone 1D10 (AY274838) | 99 | d | |
| AJ630737 | Clone 1A3 (AY274867) | 98 | c | |
| AJ630738 | Clone ARCTIC.123 (AF277538) | 96 | d | |
| AJ630739 | Clone MERTZ_OCM_182 (AF424352) | 90 | d | |
| AJ630740, AJ630741 | Clone OCS155 (AF001652) | 99 | Actinobacteria | a,b,c |
| AJ630742, AJ630743 | Clone SAa03 (AY124414) | 99 | a | |
| AJ630744 | Clone SAb10 (AY124422) | 99 | a | |
| AJ630745 | Clone FBP234 (AY250866) | 93 | b |
a
a, clone library of North Sea surface water; b, library of sorted cells after hybridization with probe EUB338; c, library of sorted cells after hybridization with probe BET42a; d, library of sorted cells after hybridization with probe CF319a.