TABLE 1.

Specific growth rate, production and consumption rates, and yields^a in P. anomala when grown aerobically, exposed to a shift in oxygen tension or to a glucose pulse^b

Parameter	Aerobic growth (glucose)	After shift to 0% oxygen (glucose)	After glucose pulse (succinate and glucose)
Specific growth rate (h−1)	0.22 ± 0.02	0.056 ± 0.009	0.21 ± 0.04
Generation time (h)	3.2 ± 0.3	12.4 ± 1.7	3.3 ± 0.5
Specific consumption or production rate (mmol g of biomass−1 h−1)
Glucose	2.1 ± 0.4	4.6 ± 0.5	2.65 ± 0.6
Ethanol	0.14c	6.2 ± 0.6	0 ± 0
Glycerol	0.12c	0.61 ± 0.06	NDd
Acetate	0.22c	0	ND
Yield (g g of glucose−1)
Biomass	0.59 ± 0.13	0.11 ± 0.04	ND
Ethanol	0.03 ± 0.004	0.48 ± 0.09	ND
Glycerol	0.05 ± 0.02	0.08 ± 0.02	ND
Acetate	0.06 ± 0.009	0 ± 0	ND
Carbon balancee (%)	80	100

^aThe yield coefficients were calculated on data from samples taken during exponential phase (aerobic culture) and at 4 to 6 h after oxygen limitation (when maximum yield of ethanol was obtained).

^bValues are averages ± standard deviations based on the results from three independent cultivations. Carbon sources are given in parentheses.

^cRate estimation from intermediate production of acetate, ethanol, and glycerol during aerobic growth.

^dND, not determined.

^eCO₂ production was not measured, but theoretical yields were calculated from ethanol production.