TABLE 3.

Oligonucleotides for detection and genotyping of F+ coliphages used in this study

Oligonucleotide identitya	Sequence (5′-3′)b	Orien- tation	Targetc
Primer
JV40	AAG AAC AGT AAR ACW GAT CG	+	3
JV41	CCC ATD GAR GAW ATY TTC TC	−	3
JV80	GAG CCY GAT ATG AAT ATG	+	2
JV81	CCA RAA DAT CAT GGA CTC	−	2
MJV82	GTA TAG AYC TKA AYG AYC A	+	2 and 3
SL2	GTA ACT TGG TAT TCA AAG CA	+	1
SL3	AAG GAG CGG AAT TAT CAT CA	−	1
Probe
M13	TAA ACC TGA AAA TCT ACG C	+	1
fd	TTC CAT AAT TCA GAA ATA TAA C	+	1
CH	ATA ATT TGC GTA ATT TCT TC	+	1
con	TTC TTY ATH TCT GTT TTA CG	+	1
MS2	GAG ACG ATA CGA TGG GAA C	+	2
GA	AAT CAG GAR TTA GCC CGA	+	2
Qb	CAC GAA GGC TCC GTT AMT AAT	+	3
M11	GGC AGC CGT GAY GAT AA	+	3
SP	GAT GGG TCT YTG CTA AAT CAT	+	3
FI	GCA GCC AGC GAC TCT AT	+	3

^aProbes are 5′ hexylamine labeled.

^bIUPAC codes used to indicate degenerate positions.

^c1, inovirus; 2, levivirus; 3, allolevivirus.