TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli | ||
| SM10 | Mobilizing strain; RP4 tra genes; Kmr | 35 |
| TOP10 | Used for cloning and blue-white screening | Invitrogen |
| S17-1 | thi pro hsdR recA; chromosomal RP4; Tra+; Smr Spr | 35 |
| A. tumefaciens bioreporter strain A136 | Lacks the Ti plasmid | 13 |
| B. thailandensis | ||
| DW503 | Derived from E264; Δ(amrR-oprA)(Kms Gms Sms); rpsL (Smr) | 4 |
| RU01 | DW503 containing pBHR1; Kmr | This study |
| RU02 | DW503 containing pBA01; Kmr | This study |
| RU03 | DW503 containing pME6000; Tcr | This study |
| RU04 | DW503 containing pME6863; Tcr | This study |
| BTRJ1 | DW503 derivative; 11::pRU11; Gmr | 38 |
| BTRJ3 | DW503 derivative; 13::pRU13; Gmr | 38 |
| BTRJ6 | DW503 derivative; R3::pRUR3; Gmr | 38 |
| Plasmids | ||
| pPCR2.1-TOPO | TA cloning vector; Kmr Apr | Invitrogen |
| pBHR1 | Mobilizable broad-host-range vector; Kmr Cmr | MoBiTee |
| pME6000 | Broad-host-range cloning vector; Tcr | 30 |
| pME6863 | pME6000 carrying the aiiA gene from B. cereus strain A24; Tcr | 30 |
| pBA01 | pBHR1 containing the B. anthracis (Ames strain) aiiA gene | This study |