TABLE 5.
Bacterial 16S rDNA clones from sedimentsa of the FRC background area borehole FWB303
| Clone ID | GenBank no. | Frequencyb | Affiliationc (% similarity) | Putative division |
|---|---|---|---|---|
| S-A1 | AY622264 | 54 | Pseudomonas agarici (98) | γ-Proteobacteria |
| S-H52 | AY622270 | 36 | Pseudomonas rhodesiae (99) | γ-Proteobacteria |
| S-E105 | AY622267 | 3 | Aquaspirillum autotrophicum (97) | β-Proteobacteria |
| S-D24 | AY622266 | 3 | Arthrobacter sp. JCM 1339 (98) | Actinobacteria |
| S-G30 | AY622269 | 1 | Duganella zoogloeoides (98) | β-Proteobacteria |
| S-B2 | AY622265 | 1 | Clone 32-20 (95) | Unknown |
| S-F26 | AY622268 | 1 | Clone 3C0d-3 (93) | Clostridia |
| S-J147 | AY622271 | 1 | Thermoleiphilum minutumd (AJ458464) | Actinobacteria |
a
Sediments were stored at 4°C for approximately 3 weeks prior to analysis.
b
Frequency of a given RFLP type out of 100 total clones.
c
Closest identified match in the GenBank database followed by percent similarity.
d
The sequence of clone S-J147 did not appear to be chimeric; however, it did not significantly match anything in the GenBank database, cultured or uncultured. Portions of the gene shared significant similarity with that of Thermoleiphilum minutum, but across the entire gene similarity was only 76%.