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Evaluation of linear plasmids under different growth conditions. Lane 1, λ ladder; lane 2, strain AJ grown on VC; lane 3, strain AJ grown on LB broth following growth on VC; lane 4, strain AJ grown on ethene; lane 5, strain AJ grown on LB broth following growth on ethene; lane 6, strain AJ grown on ethylene oxide; lane 7, strain TD grown on VC; lane 8, strain TD grown on ethene; and lane 9, Mycobacterium sp. strain JS60 grown on ethene.
