FIG. 1.

Comparison of RT-PCR and RT-booster PCR on RNA from a viral suspension. Lanes 1 to 8 (from left), 10-fold dilutions (from 10⁻¹ to 10⁻⁸) of viral RNA (patient 5 from the Turin outbreak) subjected to RT-PCR; lane 9, negative control for RT-PCR; lane 10, marker (100 bp); lanes 11 to 18, same RNA dilutions as in lanes 1 to 8 subjected to RT-booster PCR; lanes 19 and 20, negative controls for the first and second PCRs of RT-booster PCR.