Skip to main content
. 2004 Oct;70(10):6329–6332. doi: 10.1128/AEM.70.10.6329-6332.2004

FIG. 2.

FIG. 2.

Comparison of RT-PCR and RT-booster PCR on experimentally contaminated shellfish (electrophoretic gel and Southern blot). Lanes 1, 9, and 18 (from left), marker (100 bp); lanes 2 to 5, 10-fold dilutions (from 10−1 to 10−4) of NV (strain 99-23 372) in shellfish extract subjected to RT-PCR; lanes 10 to 13, 10-fold dilutions (from 10−1 to 10−4) of NV (strain 99-23 372) in shellfish extract subjected to RT-booster PCR; lanes 6 and 14, RT-PCR and RT-booster PCR on uninoculated shellfish extract; lanes 7 and 15, RT-PCR and RT-booster PCR positive controls; lane 8, RT-PCR negative control; lanes 16 and 17, RT-booster PCR negative controls for the first and second PCRs.